3EK7
Calcium-saturated GCaMP2 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.131, 47.310, 68.940 |
| Unit cell angles | 90.00, 100.48, 90.00 |
Refinement procedure
| Resolution | 20.670 - 1.850 |
| R-factor | 0.19129 |
| Rwork | 0.189 |
| R-free | 0.24052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1EMA and 1CDL |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.735 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.730 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 36788 | |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2 M lithium sulfate monohydrate, 0.1 M Tris-HCl pH 8.5, 30%(w/v) polyethylene glycol 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
