3EJZ
Structure of E203V mutant E.coli Cl-/H+ exchanger, CLC-ec1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Detector technology | CCD |
| Collection date | 2008-01-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.259, 96.415, 170.150 |
| Unit cell angles | 90.00, 131.78, 90.00 |
Refinement procedure
| Resolution | 58.760 - 2.900 |
| R-factor | 0.25338 |
| Rwork | 0.252 |
| R-free | 0.28087 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ots as a starting model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.072 | 0.540 |
| Number of reflections | 58605 | |
| <I/σ(I)> | 18.9 | 2.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 36% (w/v) PEG 300, 20 mM NaBr, 50 mM tris-SO4, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
