3EJW
Crystal Structure of the Sinorhizobium meliloti AI-2 receptor, SmLsrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.848, 71.488, 68.035 |
| Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
| Resolution | 22.461 - 1.800 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tjy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.007 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.460 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.348 | |
| Number of reflections | 130666 | |
| <I/σ(I)> | 15.33 | 2.6 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2, 30% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
