3EJV

Crystal structure of a cystatin-like protein (saro_2766) from novosphingobium aromaticivorans dsm at 1.40 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97929
Spacegroup name	P 21 3
Unit cell lengths	79.540, 79.540, 79.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.127 - 1.400
R-factor	0.143
Rwork	0.142
R-free	0.16100
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.545
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.127	28.127	1.450
High resolution limit [Å]	1.400	3.010	1.400
Rmerge	0.048	0.022	0.550
Number of reflections	33205	6443	6198
<I/σ(I)>	13.99	40.3	2
Completeness [%]	99.3	99.4	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	2.0000M (NH4)2SO4, 2.0000% PEG-400, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K