3EJI
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-22 |
| Detector | BRUKER PROTEUM |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.200, 48.200, 63.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 1.900 |
| R-factor | 0.209 |
| Rwork | 0.203 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.435 |
| Data reduction software | APEX |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.034 | 0.257 |
| Number of reflections | 11208 | |
| <I/σ(I)> | 19.93 | 3.75 |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 5.11 | 5.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 279 | 44% MPD, 25 mM Potassium Phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
