3EJH
Crystal Structure of the Fibronectin 8-9FnI Domain Pair in Complex with a Type-I Collagen Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.840, 56.840, 150.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.225 - 2.100 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24200 |
| Starting model (for MR) | PDB ENTRIES 2CG6 2cg7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.297 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.225 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.571 |
| Total number of observations | 20451 | |
| Number of reflections | 19940 | |
| <I/σ(I)> | 6.023 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.5 M NaCl, 0.1 M BisTris pH 6.5 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
