3EJG
Crystal structure of HCoV-229E X-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.560, 65.890, 38.020 |
| Unit cell angles | 90.00, 110.07, 90.00 |
Refinement procedure
| Resolution | 65.940 - 1.780 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.284 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.940 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.052 | 0.155 |
| Number of reflections | 14479 | |
| <I/σ(I)> | 9.067 | 4.4 |
| Completeness [%] | 96.9 | 89.9 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 285.15 | 20% PEG 8000, 0.1 M TRIS PH 8.5, 5% MPD, temperature 285.15K |
