3EJF
Crystal structure of IBV X-domain at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-08 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.207, 79.811, 99.741 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.820 - 1.600 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.349 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.820 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.103 | 0.244 |
| Number of reflections | 22201 | |
| <I/σ(I)> | 18.371 | |
| Completeness [%] | 97.9 | 97.1 |
| Redundancy | 7.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 285.15 | 30% PEG 4000, 0.1 M TRIS PH 8.5, 0.2 M MAGENSIUM CHLORIDE, temperature 285.15K |
