3EIQ

Crystal structure of Pdcd4-eIF4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	2008-05-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9725
Spacegroup name	P 21 3
Unit cell lengths	198.362, 198.362, 198.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.910 - 3.500
R-factor	0.2223
Rwork	0.220
R-free	0.26935
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3EIJ 1QDE and 1FUK
RMSD bond length	0.019
RMSD bond angle	2.042
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.500
Rmerge	0.108
Number of reflections	32991
<I/σ(I)>	5.8
Completeness [%]	99.8
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	20% PEG 3350, 0.1M Bis-Tris Propane pH6.5, 0.2M sodium citrate, 0.1M taurine, VAPOR DIFFUSION, HANGING DROP, temperature 288K