3EIP
CRYSTAL STRUCTURE OF COLICIN E3 IMMUNITY PROTEIN: AN INHIBITOR TO A RIBOSOME-INACTIVATING RNASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1993-08 |
Detector | SDMS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.070, 53.720, 36.250 |
Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
Resolution | 44.600 - 1.800 |
Rwork | 0.218 |
R-free | 0.26500 * |
Structure solution method | MIR |
RMSD bond length | 0.006 |
RMSD bond angle | 24.400 * |
Data reduction software | SDMS |
Phasing software | MLPHARE |
Refinement software | CNS (0.4A) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.600 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.044 | 0.167 * |
Number of reflections | 11860 * | |
<I/σ(I)> | 41.1 | 2.5 |
Completeness [%] | 81.0 * | 77 * |
Redundancy | 2.03 | 1.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 2.0 M AMMONIUM SULFATE, 0.1 M MES BUFFER, PH 6.0, pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | sodium azide | 10 (mM) | |
2 | 1 | drop | ammonium sulfate | 1.4 (M) | |
3 | 1 | drop | sodium chloride | 80 (mM) | |
4 | 1 | drop | MES | 33 (mM) | |
5 | 1 | drop | sodium azide | 0.005 (mM) | |
6 | 1 | drop | PMSF | 0.005 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 2 (M) | |
8 | 1 | reservoir | MES | 100 (mM) | |
9 | 1 | reservoir | sodium chloride | 100 (mM) |