3EI7

Crystal structure of apo-LL-diaminopimelate aminotransferase from Arabidopsis thaliana (no PLP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.115872
Spacegroup name	P 32 2 1
Unit cell lengths	103.101, 103.101, 171.827
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.720 - 1.990
R-factor	0.19125
Rwork	0.189
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z20
RMSD bond length	0.018
RMSD bond angle	1.551
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0055)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.060
High resolution limit [Å]	1.990	1.990
Number of reflections	73013
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	45% (NH4)2SO4, 0.1 M HEPES pH 7.5, 3% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K