3EI5

Crystal structure of LL-diaminopimelate aminotransferase from Arabidopsis thaliana complexed with PLP-Glu: an external aldimine mimic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978483
Spacegroup name	P 32 2 1
Unit cell lengths	102.556, 102.556, 171.953
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.470 - 2.050
R-factor	0.17303
Rwork	0.170
R-free	0.22078
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z20
RMSD bond length	0.019
RMSD bond angle	1.640
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	62726
Completeness [%]	94.5	68.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	45% (NH4)2SO4, 0.1 M HEPES pH 7.5, 3% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K