3EI0
Structure of the E221A mutant of the Gloebacter violaceus pentameric ligand gated ion channnel (GLIC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-05-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.638, 133.765, 161.647 |
| Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
| Resolution | 19.997 - 3.500 |
| R-factor | 0.256 |
| Rwork | 0.255 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.047 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.131 | 0.544 |
| Number of reflections | 46926 | |
| <I/σ(I)> | 16.1 | 2.6 |
| Completeness [%] | 99.1 | |
| Redundancy | 6.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277.15 | 9% PEG4000, 50mM CH3COONa, 500mM (NH4)2SO4, pH4.0, vapor diffusion, hanging drop, temperature 277.15K, VAPOR DIFFUSION, HANGING DROP |
