3EHZ
X-ray structure of the pentameric ligand gated ion channel of Gloebacter violaceus (GLIC) in a presumptive open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-05-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 179.225, 132.864, 161.422 |
Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
Resolution | 19.972 - 3.100 |
R-factor | 0.239 |
Rwork | 0.238 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vl0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.378 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 48.850 | 3.270 |
High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
Rmerge | 0.092 | 0.037 | 0.607 |
Total number of observations | 12519 | 62851 | |
Number of reflections | 66826 | ||
<I/σ(I)> | 5.28 | 10.1 | 1.1 |
Completeness [%] | 99.6 | 98 | 99.9 |
Redundancy | 6.3 | 5.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277.15 | 9% PEG4000, 50mM CH3COONa, 200mM (NH4)2SO4, pH4.0, vapor diffusion, hanging drop, temperature 277.15K, VAPOR DIFFUSION, HANGING DROP |