3EHV
X-ray structure of human ubiquitin Zn(II) adduct
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-26 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.357, 50.946, 93.317 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.150 - 1.810 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.500 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.010 | 0.081 |
Number of reflections | 19094 | |
<I/σ(I)> | 11.7 | 1.3 |
Completeness [%] | 94.8 | 30.9 |
Redundancy | 3.7 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25% (w/v) PEG 1450, 50mM HEPES pH 7.0, 25mM zinc acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |