3EHR
Crystal Structure of Human Osteoclast Stimulating Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.306, 57.935, 60.477 |
| Unit cell angles | 61.75, 76.53, 90.08 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.181 |
| Rwork | 0.178 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.037 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.367 | 22.820 | 2.060 |
| High resolution limit [Å] | 1.950 | 6.170 | 1.950 |
| Rmerge | 0.044 | 0.024 | 0.150 |
| Total number of observations | 1468 | 6316 | |
| Number of reflections | 21394 | ||
| <I/σ(I)> | 11.634 | 22.4 | 4.8 |
| Completeness [%] | 89.9 | 96.8 | 91.2 |
| Redundancy | 2 | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% PEG20000, 0.1M MES, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
