3EHK
Crystal structure of Pru du amandin, an allergenic protein from prunus dulcis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 151.099, 151.099, 164.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.310 - 3.200 |
| R-factor | 0.25679 |
| Rwork | 0.254 |
| R-free | 0.27899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ud1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.891 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.137 | 0.310 |
| Number of reflections | 59067 | |
| <I/σ(I)> | 4.1 | 1.7 |
| Completeness [%] | 96.6 | 98.4 |
| Redundancy | 2.56 | 2.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Tris-HCl (pH8.5), 2.0M Ammonium Sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
