3EHJ
Crystal structure of DesKC-H188V in complex with AMP-PCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.300, 34.300, 131.200 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.057 | 0.251 |
| Number of reflections | 19539 | |
| <I/σ(I)> | 9.86 | 3.1 |
| Completeness [%] | 96.3 | 96.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | PEG3350, CaCl2, bicine, ADP, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
