3EHE
Crystal structure of UDP-glucose 4 epimerase (galE-1) from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.574, 55.529, 89.634 |
Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
Resolution | 34.460 - 1.870 |
R-factor | 0.253 |
Rwork | 0.253 |
R-free | 0.28700 |
Structure solution method | SAD |
Starting model (for MR) | None |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.940 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.060 | 0.381 |
Number of reflections | 54749 | |
<I/σ(I)> | 12.1 | 1 |
Completeness [%] | 99.7 | 97.8 |
Redundancy | 7.5 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | 25% Ethylene Glycol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |