3EHB
A D-Pathway Mutation Decouples the Paracoccus Denitrificans Cytochrome c Oxidase by Altering the side chain orientation of a distant, conserved Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92021 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.461, 151.332, 157.487 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.320 |
| R-factor | 0.20618 |
| Rwork | 0.205 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ar1 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.488 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | DM |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.090 | |
| Number of reflections | 87146 | |
| <I/σ(I)> | 19.14 | 3.93 |
| Completeness [%] | 98.8 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | MPEG 2000 is contained in the crystallization buffer pH 5.5, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
