3EH2
Crystal structure of the human COPII-coat protein Sec24c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.690, 182.980, 201.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.780 - 2.350 |
| Rwork | 0.241 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.047 | 0.346 |
| Number of reflections | 104257 | |
| <I/σ(I)> | 27.8 | 3.6 |
| Completeness [%] | 99.4 | 98.3 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | CRYSTALS WERE GROWN VIA THE HANGING-DROP METHOD, BY ADDING 1UL PROTEIN SOLUTION TO 1UL OF WELL SOLUTION COMPRISING 6% (W/V) PEG 4000 AND 0.1 M MES., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
