3EGJ
N-acetylglucosamine-6-phosphate deacetylase from Vibrio cholerae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 77.001, 77.001, 282.549 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.200 - 2.900 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yrr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.767 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.200 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.128 | 0.732 |
| Number of reflections | 19809 | |
| <I/σ(I)> | 21.618 | 3.71 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 10.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M ammonium sulfate, 0.1 M Tris, 25% w/v Polyethylene glycol 3,350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
