3EGC
Crystal structure of a putative ribose operon repressor from Burkholderia thailandensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.712, 288.651, 63.355 |
| Unit cell angles | 90.00, 113.59, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.29900 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.652 |
| Data reduction software | DENZO |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.474 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.090 | 0.165 |
| Total number of observations | 73821 | |
| Number of reflections | 67644 | |
| <I/σ(I)> | 20.6 | 8.9 |
| Completeness [%] | 98.0 | 99.9 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 294 | 100mM tri-sodium citrate pH 5.6, 10% PEG MME 5K, 100mM magnesium acetate, Vapor diffusion, temperature 294K |
