3EGA
Crystal structure of Pellino2 FHA Domain at 1.8 Angstroms resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795, 0.9796, 0.9495 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 83.650, 86.137, 162.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.070 - 1.800 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.24400 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.510 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.100 | 0.515 |
Number of reflections | 27082 | |
<I/σ(I)> | 37.257 | 4 |
Completeness [%] | 98.5 | 86.9 |
Redundancy | 8.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 295 | 0.1 M sodium acetate, 26.4% w/v PEG 2000 MME, 0.2 M ammonium sulfate, pH 5.5, vapor diffusion, temperature 295K |