3EFV
Crystal Structure of a Putative Succinate-Semialdehyde Dehydrogenase from Salmonella typhimurium LT2 with bound NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 133.826, 133.826, 245.276 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.352 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.422 | |
| Number of reflections | 189522 | |
| <I/σ(I)> | 8.5 | 5.73 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 298 | 2M Na Formate, 0.1M Na Acetate pH4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
