3EFM
Structure of the alcaligin outer membrane recepteur FauA from Bordetella pertussis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | FLAT PANEL |
Collection date | 2008-05-16 |
Wavelength(s) | 0.9789 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 165.893, 188.847, 62.437 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.350 - 2.330 |
R-factor | 0.22091 |
Rwork | 0.219 |
R-free | 0.25843 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o5p |
RMSD bond length | 0.015 |
RMSD bond angle | 1.565 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.420 | 2.400 |
High resolution limit [Å] | 2.330 | 2.330 |
Number of reflections | 42320 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.75% C8E4, 0.1M HEPES, pH7.5, 0.3-0.5M NH4SO4, 13-17% MPD, protein concentration 10mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 293K |