3EFG

Structure of SlyX protein from Xanthomonas campestris pv. campestris str. ATCC 33913

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97931, 0.91966, 0.91948
Spacegroup name	P 63
Unit cell lengths	35.945, 35.945, 81.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.130 - 2.000
R-factor	0.204
Rwork	0.204
R-free	0.21100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.389
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.051	0.533
Number of reflections	4751
<I/σ(I)>	58.469
Completeness [%]	99.7	100
Redundancy	12.4	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M Tris pH 8.5, 0.2M Ammonium Sulfate, 20% PEG 3350, 4% Glycerol, 1/60 cymotrypsin, then soaked in Na Bromide for phasing, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M Tris pH 8.5, 0.2M Ammonium Sulfate, 20% PEG 3350, 4% Glycerol, 1/60 cymotrypsin, then soaked in Na Bromide for phasing, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1M Tris pH 8.5, 0.2M Ammonium Sulfate, 20% PEG 3350, 4% Glycerol, 1/60 cymotrypsin, then soaked in Na Bromide for phasing, VAPOR DIFFUSION, HANGING DROP, temperature 291K