3EFA
Crystal structure of putative N-acetyltransferase from Lactobacillus plantarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 84.673, 84.673, 174.074 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.877 - 2.423 |
R-factor | 0.2077 |
Rwork | 0.206 |
R-free | 0.23660 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.156 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.112 | 0.537 |
Number of reflections | 14578 | |
<I/σ(I)> | 48.35 | 6.9 |
Completeness [%] | 99.1 | 100 |
Redundancy | 12.2 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M Bis-TRIS 0.5M Magnesium formate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |