3EF9
Replacement of Val3 in Human Thymidylate Synthase Affects Its Kinetic Properties and Intracellular Stability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Collection date | 2008-06-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.349, 96.349, 83.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| Rwork | 0.252 |
| R-free | 0.29800 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.195 | |
| Number of reflections | 6685 | |
| <I/σ(I)> | 5.364 | |
| Completeness [%] | 75.5 | 22.6 |
| Redundancy | 2.5 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M Tris,PH8.5, 18%PEG4000,60mM ammonium sulfate, 20mM BME, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
