3EF1

The Structure of Fcp1, an essential RNA polymerase II CTD phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	53.663, 88.442, 113.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
Rwork	0.211
R-free	0.23400
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.059	0.340
Number of reflections	29320
<I/σ(I)>	19.043	1.7
Completeness [%]	97.4	94.7
Redundancy	4.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	Fcp1-BeF3-Mg was obtained by incubating 200 M Fcp1(140-580) with 225 M CTD peptide (SPSYSPTSPS), 300 M BeCl2, 5 mM NaF and 5 mM MgCl2 on ice for 1 h then crystallized by sitting drop vapor diffusion against 22% PEG-4000, 100 mM Na-citrate (pH 5.6), 100 mM ammonium acetate, 5% hexane 1,6-diol, 5% aminocaproic acid, 5% propane-1,3-diol, 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 291K