3EEY
CRYSTAL STRUCTURE OF PUTATIVE RRNA-METHYLASE FROM Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.156, 126.913, 125.611 |
| Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.24468 |
| Rwork | 0.244 |
| R-free | 0.27843 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 | 0.600 |
| Number of reflections | 109977 | |
| <I/σ(I)> | 5.5 | 0.8 |
| Completeness [%] | 99.2 | 98.3 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 294 | 100MM SODIUM ACETATE, PH 4.5, 30% PEG8000, 200MM LITHIUM SULFATE, 10% GLYCEROL, 294K, pH 4.50 |
