3EEX
The crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.502, 82.401, 109.428 |
| Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.490 |
| R-factor | 0.26578 |
| Rwork | 0.262 |
| R-free | 0.29875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cka |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.094 | 0.616 |
| Number of reflections | 22424 | |
| <I/σ(I)> | 2.33 | |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 34% PEG400, 0.1M Tris-HCl pH 9, protein 20.2 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
