3EET
Crystal structure of putative GntR-family transcriptional regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 87.157, 87.157, 123.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.198 - 1.969 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21820 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.969 | 1.970 |
| Rmerge | 0.079 | 0.665 |
| Number of reflections | 74259 | |
| <I/σ(I)> | 48.8 | 3.45 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.7 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 0.2M Na formate,20%PEG3350, 1/400 thermolysin w/w, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
