3EES
Structure of the RNA pyrophosphohydrolase BdRppH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-09 |
Detector | adsc Q210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.491, 70.491, 100.481 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.260 - 1.900 |
R-factor | 0.223 |
Rwork | 0.222 |
R-free | 0.25600 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.298 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.03) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.260 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.043 | 0.033 | 0.390 |
Number of reflections | 23316 | ||
<I/σ(I)> | 63.364 | ||
Completeness [%] | 99.8 | 98 | 100 |
Redundancy | 10 | 10 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 4 | 298 | PEG 4000, Na acetate, hanging drop, temperature 298K |