3EEQ
Crystal structure of a putative cobalamin biosynthesis protein G homolog from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.015, 97.500, 107.322 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.28400 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.734 |
| Data reduction software | DENZO |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.737 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.079 | 0.593 |
| Total number of observations | 66671 | |
| Number of reflections | 31415 | |
| <I/σ(I)> | 25.2 | 4.6 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 14.5 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 294 | 100mM sodium acetate pH 4.5, 19% PEG 4K, 200mM ammonium sulfate, Vapor diffusion, temperature 294K |
