3EEG
Crystal structure of a 2-isopropylmalate synthase from Cytophaga hutchinsonii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2008-07-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 118.666, 118.666, 154.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.950 - 2.780 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.29800 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.880 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.155 | 0.790 |
Number of reflections | 16841 | |
<I/σ(I)> | 6.2 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.1 | 20.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES 7.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |