3EEE
Probing the function of heme distortion in the H-NOX family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.253, 124.686, 83.555 |
| Unit cell angles | 90.00, 126.58, 90.00 |
Refinement procedure
| Resolution | 45.672 - 2.120 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.25190 |
| Starting model (for MR) | 1u55 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.898 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.055 | 0.455 |
| Number of reflections | 53717 | |
| <I/σ(I)> | 2.7 | |
| Redundancy | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.1 | 289 | 0.1 M NaSCN, 0.1 M Tris pH 9.1, 0.2 M (NH4)2(SO4), and 18% (w/v) PEG 8000 at 289K |
