3EE1
Novel fold of VirA, a type III secretion system effector protein from Shigella flexneri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.252, 170.910, 46.165 |
| Unit cell angles | 90.00, 104.89, 90.00 |
Refinement procedure
| Resolution | 28.631 - 3.010 |
| R-factor | 0.2157 |
| Rwork | 0.214 |
| R-free | 0.25560 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.529 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.082 | 0.060 | 0.421 |
| Number of reflections | 21471 | ||
| Completeness [%] | 96.8 | 99.9 | 80.5 |
| Redundancy | 7.1 | 7.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
