3EDP
The crystal structure of the protein lin2111 (functionally unknown) from Listeria innocua Clip11262
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.345, 66.620, 100.751 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 43.367 - 2.092 |
| R-factor | 0.2021 |
| Rwork | 0.200 |
| R-free | 0.24240 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.879 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | 2.140 |
| High resolution limit [Å] | 2.092 | 2.100 |
| Rmerge | 0.109 | 0.825 |
| Number of reflections | 75991 | |
| <I/σ(I)> | 20.6 | 1.6 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 4.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1M Tri-sodium citrate 0.1M Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
