3EDI
Crystal structure of tolloid-like protease 1 (TLL-1) protease domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.281, 58.923, 47.444 |
Unit cell angles | 90.00, 104.62, 90.00 |
Refinement procedure
Resolution | 29.960 - 1.400 |
R-factor | 0.155 |
Rwork | 0.154 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dle |
RMSD bond length | 0.016 |
RMSD bond angle | 1.473 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.900 | 1.440 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.073 | 0.479 |
Number of reflections | 41793 | |
<I/σ(I)> | 15.9 | 2.9 |
Completeness [%] | 96.3 | 74.1 |
Redundancy | 3.5 | 2.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | 25% PEG 3350, 200mM lithium sulphate, 100mM Bis-Tris-HCl, pH 7.5, vapor diffusion, temperature 277K |