3EDG
Crystal structure of bone morphogenetic protein 1 protease domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.432, 57.260, 68.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.020 - 1.270 |
R-factor | 0.146 |
Rwork | 0.144 |
R-free | 0.17700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3edi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.375 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.790 | 1.310 |
High resolution limit [Å] | 1.270 | 1.270 |
Rmerge | 0.091 | 0.471 |
Number of reflections | 57443 | |
<I/σ(I)> | 17.1 | 2.9 |
Completeness [%] | 98.3 | 86.3 |
Redundancy | 6.6 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | pH 7.5, vapor diffusion, temperature 277K |