3ED3
Crystal Structure of the Yeast Dithiol/Disulfide Oxidoreductase Mpd1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.157, 92.268, 79.337 |
| Unit cell angles | 90.00, 92.23, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.219 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.284 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 45863 | |
| <I/σ(I)> | 17 | 3.3 |
| Completeness [%] | 97.9 | 94.7 |
| Redundancy | 5.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 17% PEG4K, 0.2M sodium acetate, 100mM cacodylic acid (pH5.9), 6-10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 17% PEG4K, 0.2M sodium acetate, 100mM cacodylic acid (pH5.9), 6-10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
