3ED1
Crystal Structure of Rice GID1 complexed with GA3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-07-18 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.717, 134.142, 118.872 |
Unit cell angles | 90.00, 105.20, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.19925 |
Rwork | 0.197 |
R-free | 0.23946 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ebl |
RMSD bond length | 0.013 |
RMSD bond angle | 1.488 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.054 | 0.365 |
Number of reflections | 192246 | |
<I/σ(I)> | 27.585 | |
Completeness [%] | 97.6 | 87.5 |
Redundancy | 3.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15% PEG4000, 8% MPD, 0.2M NaNO3, 0.1M HEPES pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |