3ECT
Crystal Structure of the Hexapeptide-Repeat Containing-Acetyltransferase VCA0836 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-10 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 73.319, 73.319, 178.515 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.260 - 2.510 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.620 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.260 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.751 |
| Number of reflections | 6617 | |
| <I/σ(I)> | 6 | 2.16 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.2 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 0.02 M Calcium Chloride, 0.1 M Sodium Acetate, 30% MPD, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
