3ECO

Crystal structure of MepR, a transcription regulator of the Staphylococcus aureus multidrug efflux pump MepA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Wavelength(s)	0.9797 0.9798 1.02
Spacegroup name	P 21 21 21
Unit cell lengths	32.196, 96.574, 110.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.890 - 2.400
Rwork	0.242
R-free	0.28900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	500.000
High resolution limit [Å]	2.400
Rmerge	0.046
Number of reflections	14174
<I/σ(I)>	25.6
Completeness [%]	99.8
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG 4000, 0.2 M Magnesium sulfate,10% glycerol, NDSB-256, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG 4000, 0.2 M Magnesium sulfate,10% glycerol, NDSB-256, VAPOR DIFFUSION, HANGING DROP, temperature 293K