3ECC

Crystal structure of the DnaC helicase loader in complex with ADP-BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-07
Detector	ADSC QUANTUM 210
Spacegroup name	P 61
Unit cell lengths	84.582, 84.582, 49.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.263
Rwork	0.263
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	ELVES

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.000	2.230
High resolution limit [Å]	2.120	2.120
Number of reflections	5408
<I/σ(I)>	11.6	1.8
Redundancy	3.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	294	50mM Tris, 1% PEG 6000, 1mM TCEP, 2mM ADP, 5mM MgCl2, 4mM BeCl2, 12mM NaF, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K