3EC6
Crystal structure of the General Stress Protein 26 from Bacillus anthracis str. Sterne
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-19 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.123, 48.898, 51.066 |
| Unit cell angles | 90.00, 126.05, 90.00 |
Refinement procedure
| Resolution | 26.320 - 1.600 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.842 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.270 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.276 |
| Number of reflections | 16512 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 96.2 | 71.4 |
| Redundancy | 3.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 295 | 0.1M Hampton PH9.2, 2.8M NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
