3EC2
Crystal structure of the DnaC helicase loader
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795, 1.11587 |
| Spacegroup name | P 61 |
| Unit cell lengths | 84.492, 84.492, 51.113 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| Rwork | 0.233 |
| R-free | 0.27800 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.985 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 5534 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 50mM Tris, 1% PEG 6K, 1mM TCEP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
