3EC1
Structure of YqeH GTPase from Geobacillus stearothermophilus (an AtNOS1 / AtNOA1 ortholog)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-29 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.615, 81.097, 108.173 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.360 |
| Rwork | 0.254 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 34381 | |
| <I/σ(I)> | 21.79 | 4.13 |
| Completeness [%] | 99.4 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | Protein (10mg/ml) was mixed with equal volume of well solution, that contained 100 mM Sodium Citrate pH 5.0 - 5.6, 0-5% PEG 5K MME, with 500 microliters of well solution, VAPOR DIFFUSION, temperature 298K |
