3EBV
Crystal structure of putative Chitinase A from Streptomyces coelicolor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.636, 96.636, 124.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.210 - 1.500 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.16900 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.463 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.088 | 0.454 |
| Number of reflections | 70137 | |
| <I/σ(I)> | 22.1 | 4.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | 0.1 M CAPS pH 10.5, 2.0 M Ammonium sulfate, 0.2 M Lithium sulfate, Vapor diffusion, Sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
